Coding

Part:BBa_K2020043:Design

Designed by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher   Group: iGEM16_Aachen   (2016-10-14)


AzF-Synthetase for use in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 84
    Illegal SapI.rc site found at 877


Design Notes

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.

[http://2016.igem.org/Team:Aachen Team Aachen 2016] used similar synthetases in pACYC with p15A-ORI and Gent-Resistance. Together with a tRNA (BBa_K2020042) with own Ipp-Promoter and Terminator.



Source

Obtained via iGEM Team Austin, Texas 2014

References